Figure 4.

Conditioned medium induces expression of COX-2 and prostaglandin Fsecretion. Expression of (A) COX-1 and (B) COX-2 mRNA in HUVECs incubated with V CM or P CM for 1,2,3,4,6,16 and 24 hrs as determined by quantitative RT-PCR analysis. (C) COX-2 mRNA in HUVECs incubated with V CM or P CM for 3 hrs in the absence/presence of FGFR1 tyrosine kinase inhibitor SU4984 or ERK1/2 inhibitor PD98059 as determined by quantitative RT-PCR analysis. Data are expressed as fold increase over control V CM (where V CM = 1, not shown). HUVECs were incubated with V CM or P CM for 0, 3, 6 and 16 hrs and thereafter conditioned medium from HUVECs was collected and used for PGE2 (D) and PGF(E) ELISA analysis as described in the methods. (F) HUVECs were treated with V CM, P CM or P CM and NS398 or indomethacin (Indometh.) for 6 hrs after which the concentration of PGFin the HUVEC medium was determined by ELISA. Data are represented as mean ± SEM. (* represents statistical significance P < 0.05).

Keightley et al. BMC Cell Biology 2010 11:8   doi:10.1186/1471-2121-11-8
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