The effect of PGF2α conditioned medium on endothelial network formation and proliferation. (A) Representative image from one network formation assay in which HUVECs, plated on growth factor reduced matrigel, were incubated with V CM, P CM or P CM and SU4984 or FGF2 immunoneutralised CM (FGF2-Ab) for 16 hrs as described in the Methods. (B) Quantification of endothelial network formation and (C) proliferation using HUVECs stimulated with V CM, P CM or P CM in the presence of FGFR1 tyrosine kinase inhibitor (P CM + SU4984) or FGF2 immunoneutralised CM (P CM + FGF2Ab). Data are expressed as percentage increase over control V CM (where V CM = 100%, not shown) and presented as mean ± SEM. (* represents statistical significance P < 0.05).
Keightley et al. BMC Cell Biology 2010 11:8 doi:10.1186/1471-2121-11-8