Figure 1.

The effect of PGF_2α conditioned medium on endothelial network formation and proliferation. (A) Representative image from one network formation assay in which HUVECs, plated on growth factor reduced matrigel, were incubated with V CM, P CM or P CM and SU4984 or FGF2 immunoneutralised CM (FGF2-Ab) for 16 hrs as described in the Methods. (B) Quantification of endothelial network formation and (C) proliferation using HUVECs stimulated with V CM, P CM or P CM in the presence of FGFR1 tyrosine kinase inhibitor (P CM + SU4984) or FGF2 immunoneutralised CM (P CM + FGF2Ab). Data are expressed as percentage increase over control V CM (where V CM = 100%, not shown) and presented as mean ± SEM. (* represents statistical significance P < 0.05).