Staining of hAFS cell-derived multi-lineage cells. A clonal hAFS cell line at passage 12 derived by the starter cell method was in vitro induced with proper induction mediums and shows the appearance of lineage-specific signals of osteoblasts by staining with Alkaline Phosphatase (A) and Von Kossa (B). The lineage-specific signals of adipocytes and neurons exhibit after staining with Oil Red O (C) and Tuj-1 (D), respectively. 20× magnification was used.
Phermthai et al. BMC Cell Biology 2010 11:79 doi:10.1186/1471-2121-11-79