Figure 1.

Derivation of clonal hAFS cell lines by the starter cell method. (A) and (B) A hAFS starter cell of fibroblastic type (arrow) was found early after a routine amniocentesis culture, (C) the colony appearance of a clonal hAFS cell line at 48 h in the primary culture dish, (D) morphology of hAFS cells derived by the starter cell method at subculture passage 3.