Co-transfection of LKB1 and STRAD leads to actin puncta associated with phospho-ezrin. Panels 1 & 2: HeLa-S3 cells were transfected with myc-LKB1 and pcDNA-HisMax-STRAD. Cells were stained with anti-myc or anti-Xpress antibodies. LKB1 partially relocated into the cytosol upon STRAD expression. Panels 3-5: Cells were transfected with myc-LKB1 and pcDNA-HisMax-STRAD and visualized with anti-myc antibody, anti-phospho-ezrin antibody and phalloidin to visualize actin. Phosphorylated ezrin is associated with actin punctas in transfected cells. Panels 6-8: Cells were transfected with myc-LKB1 and pcDNA-HisMax-STRAD and visualized with anti-myc antibody and anti-phospho-ezrin antibody. The basal level of phosphorylated ezrin can be seen in untransfected cells (white arrows, panel 7). Panel 9: Z-section image of cells in panel 7 showing puncta of phosphorylated ezrin present on dorsal surface. Scale bar = 20 μm.
Xu et al. BMC Cell Biology 2010 11:77 doi:10.1186/1471-2121-11-77