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Open Access Highly Accessed Research article

A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media

Zong-Yun Tsai1, Sher Singh2, Sung-Liang Yu3, Chi-Hsien Chou4 and Steven Shoei-Lung Li156*

Author Affiliations

1 Stem Cell Laboratory, Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan

2 Department of Life Science, College of Science, National Taiwan Normal University, Taipei 116, Taiwan

3 Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei 100, Taiwan

4 Center for Resources, Research and Development, Kaohsiung Medical University, Kaohsiung 807, Taiwan

5 Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan

6 Correspondence to: Steven Shoei-Lung Li at Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan

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BMC Cell Biology 2010, 11:76  doi:10.1186/1471-2121-11-76

Published: 12 October 2010

Additional files

Additional file 1:

Fig. S1. The OCT4 and NANOG staining of T3/HDF and T3/CMHDF, as well as T3/MEF and T3/CMMEF, cells. T3/HDF and T3/CMHDF cells were grown on T3HDF feeder and feeder-free Matrigel in T3HDF-conditioned medium for 14 and 8 passages, respectively. The T3/MEF and T3/CMMEF cells were grown on MEF feeder and feeder-free on Martigel in MEF-conditioned medium for 14 and 12 passages, respectively.

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Additional file 2:

Table S1A. 102 genes abundantly differentially expressed in T3/HDF cells.

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Additional file 3:

Table S1B. 84 genes abundantly differentially expressed in T3/CMHDF cells.

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Additional file 4:

Fig. S2. Hierachical clustering (left) and principle component analysis (right) of all microarray data.

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Additional file 5:

Table S2. The expression levels and fold-changes of 21 "stemness" genes and 9 differentiation markers in T3/HDF, T3/CMHDF, T3/MEF and T3/CMMEF cells.

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Additional file 6:

Fig. S3. The top 10 GO process networks of the abundantly expressed genes among T3/HDF, T3/CMHDF, T3/MEF and T3/CMMEF cells. The common genes are indicated by blue/white strips. The white area denotes similar genes in which three of four are the same. The unique genes are marked as color bands: T3/HDF, orange; T3/CMHDF, blue; T5/MEF, red; T3/CMMEF, green.

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Additional file 7:

Table S3. The expression levels and fold-changes of 365 miRNAs in T3/HDF and T3/CMHDF cells.

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Additional file 8:

Fig. S4A. Comparison of protein spots on 2D-gels between T3/HDF and T3/CMHDF cells. Green, match spots; red, unmatch spots.

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Additional file 9:

Fig. S4B. Comparison of protein spots on 2D-gels between T3/MEF and T3/CMMEF cells. Green, match spots; red, unmatch spots.

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Additional file 10:

Fig. S4C. Comparison of protein spots on 2D-gels between T3/HDF and T3/MEF cells. Green, match spots; red, unmatch spots.

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Additional file 11:

Fig. S4D. Comparison of protein spots on 2D-gels between T3/CMHDF and T3/CMMEF cells. Green, match spots; red, unmatch spots.

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Additional file 12:

Fig. S5. The relationship between the similarities (%) of protein match spots and correlation coefficients (r) of mRNAs.

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