Figure 5.

Effects of overexpression and repression of CTDSPL2 on globin gene expression in CD34+ HPCs. (A) Detection of overexpression and repression of CTDSPL2 gene in CD34+ cells by real-time PCR. (B) Relative expression of globin genes in CD34+ cells derived from UCB. (C) Relative expression of globin genes in CD34+ cells derived from UCB 7 days after Epo-induced erythroid differentiation. (D) Real-time PCR analysis of globin gene expression in CD34+ cells overexpressing or repressing CTDSPL2. (E) Real-time PCR analysis of globin genes in EPO-induced CD34+ cells overexpressing or repressing CTDSPL2. C1, Over, C2, RNAi represent the CD34+ cells infected respectively with lentiviruses carrying the blank pWPXL vector, recombinant pWPXL-CTDSPL2, blank pLVTHM vector and recombinant pLVTHM-CTDSPL2RNAi.

Ma et al. BMC Cell Biology 2010 11:75   doi:10.1186/1471-2121-11-75
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