Figure 2.

Overexpression of CTDSPL2 increases ε- and γ- globin gene expression in K562 cells. (A) Overexpression of CTDSPL2 mRNA in stable K562/pc3.1-CTDSPL2 transfectant pools as analyzed by real-time PCR. (B) Overexpression of CTDSPL2 protein in stable K562/pc3.1-CTDSPL2 transfectant pools as analyzed by Western blot. (C) Hemoglobin-containing cells were detected by benzidine staining during hemin-induced erythroid differentiation of K562 cells. The data were obtained from three independent experiments and the error bars represent standard deviation. (D) Real-time PCR analysis of α-, ζ-, ε-, γ- and β-globin genes mRNA expression in K562 cells, K562/pc3.1 transfectants and K562/pc3.1-CTDSPL2 transfectants. (E) Real-time PCR analysis of α-, ζ-, ε-, γ- and β-globin mRNA expression in K562 cells, stable K562/pc3.1 transfectants and K562/pc3.1-CTDSPL2 transfectants 72 h after treatment with hemin. K562 and pc3.1 represent K562 cells and stable K562/pc3.1 transfectant pool respectively. A, B, C, D and E represent the five stable K562/pc3.1-CTDSPL2 transfectant pools respectively.