CTDSPL2 mRNA levels in erythroid cultures from BM and UCB and during erythroid differentiation of K562 cells and CD34+ HPCs. (A) Differential expression of CTDSPL2 in erythroid cultures from healthy adult bone marrow (BM) and umbilical cord blood (UCB) as measured by DDRT-PCR (top) and real-time PCR (bottom). (B) CTDSPL2 mRNA expression at different days during EPO-induced CD34+ cell erythroid differentiation as determined by real-time PCR. (C) Northern blot analysis of CTDSPL2 mRNA expression in K562 cells 48 h after treatment with butyrate and hemin. (D) Real-time PCR analysis of CTDSPL2 mRNA expression at different time points during hemin-induced K562 cell erythroid differentiation.
Ma et al. BMC Cell Biology 2010 11:75 doi:10.1186/1471-2121-11-75