Promoters activation in EADSCs. A) Transiently transfected EADSCs with constructs containing promoter in front of the luciferase reporter gene or pGL3 empty vector (diagrams not on scale) as a control, at 24 h post transfection. The results are expressed as the mean relative Luciferase units after normalizing with efficiency of transfection. Each reaction was done in quadruplicate, and each point represents the average ± standard deviation from three experiments. B) Efficiency of EADSCs transfection obtained with different transfection methods, using pEGFP-C1 as a reporter plasmid. Each transfection method was done in quadruplicate, and each point represents the average ± standard deviation from three experiments. EGFP was monitored by fluorescence microscope and quantified by flow cytometry.
Donofrio et al. BMC Cell Biology 2010 11:73 doi:10.1186/1471-2121-11-73