Additional file 1.

Figure 1 (supplement) showed that overexpression of EGFP or Hsp90a1 had not effect on thick filament organization. A. DNA constructs expressing EGFP or myc-tagged Hsp90a1 in skeletal muscles of zebrafish embryos were directed using the smyd1 promoter. Myosin thick filament organization was analyzed by F59 staining. B-D. Single staining with anti-myosin (F59) antibody (red) shows that expression of GFP (B) has no effect on myosin thick filament organization (C, D). E-G. Double staining shows the expression of myc-tagged Hsp90a1 (E) in a single fiber and its lack of effect on myosin thick filament organization (F, G). G represents the merged image of E and F. Scale bars = 20 mm.

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Bernick et al. BMC Cell Biology 2010 11:70   doi:10.1186/1471-2121-11-70