Short-term effect of cAMP and its derivates on formation of tight junctions. Cells were stimulated for 24 h with different cAMP derivates, stained for tight junction-associated proteins ZO-1, OCLN, CLDN5 and Jam-A and analyzed by confocal laser microscopy. The nucleus was counterstained with DAPI. Depicted are representative micrographs of at least three independent experiments using HUVEC from different donors. Scale bar represents 25 μm.
Beese et al. BMC Cell Biology 2010 11:68 doi:10.1186/1471-2121-11-68