Table 1

Effect of protein degradation inhibitors on intracellular PC levels.

Treatments

Intracellular WT PC (%)

Intracellular A267T PC (%)


Control

100

100


Lactacystin (10 μM), n = 8

119.8 ± 3.3*

131.8 ± 4.4*


Bafilomycin (100 nM), n = 9

112.6 ± 4.9

106.7 ± 7.2


Transient transfected CHO-K1 cells expressing WT or A267T PC were incubated with medium containing 10 μM lactacystin or 100 nM bafilomycin for 24 and 8 h, respectively. PC protein levels were measured in cell lysates and results are calculated as percentage of controls (samples not treated with protein degradation inhibitors). Data are presented as mean ± SEM. *p < 0.05 (Wilcoxon test).

Tjeldhorn et al. BMC Cell Biology 2010 11:67   doi:10.1186/1471-2121-11-67

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