Figure 6.

MIF is involved in hypoxia-induced proliferation and migration of cultured HUASMCs. Growth-arrested HUASMCs were transfected with wild type HIF-1α, HIF-1α-siRNA, MIF-siRNA or scrambled siRNA for 24 h and then exposed to normoxia or hypoxia (3% O₂) for 24 h. (A) Cell proliferation measured by BrdU assay (n = 6 in each group). * indicates P < 0.05 vs control cells under normoxia. # P < 0.05 vs control or scrambled siRNA transfection under hypoxia. (B) Migration of cultured HUASMCs (n = 3 in each group). * indicates P < 0.05 vs control cells under normoxia. # P < 0.05 vs control or scrambled siRNA transfection under hypoxia.