Recovery of casein micelles after incubation in conservative conditions. Aliquots of the aqueous phase of rat milk were diluted and incubated in conservative (C) or non-conservative (NC) buffers. A. Samples were processed for negative staining electron microscopy. An undiluted aliquot of the aqueous phase of rat milk was directly analyzed (Cont). Scale bar: 0.2 μm. B. Samples in conservative or non-conservative buffers were centrifuged. Supernatants (S) and pellets (P) were analyzed by SDS-PAGE followed by Coomassie Blue staining. Relative molecular masses (kDa) are indicated on the right. Data are representative of at least three independent experiments. αS1-cas: αS1-casein; β-cas: β-casein; κ-cas: κ-casein; γ-cas: γ-casein.
Le Parc et al. BMC Cell Biology 2010 11:65 doi:10.1186/1471-2121-11-65