KPNA7 localizes to the nucleus in HeLa cells. (A) Immunofluorescence microscopy of endogenous KPNA2 and KPNA7 in HeLa cells shows that KPNA7 is nuclear at steady state. (B) HA-KPNA2 and HA-KPNA7 transfected into HeLa cells, and detected with an anti-HA antibody by immunofluorescence microscopy. (C) Immunoblot of HeLa lysates probed with purified anti-KPNA7, or anti-KPNA7 in the presence of excess KPNA7 peptide to show competition for antibody binding.
Kelley et al. BMC Cell Biology 2010 11:63 doi:10.1186/1471-2121-11-63