Open Access Research article

Octamer-binding factor 6 (Oct-6/Pou3f1) is induced by interferon and contributes to dsRNA-mediated transcriptional responses

Elisabeth Hofmann1, Ursula Reichart1, Christian Gausterer15, Christian Guelly2, Dies Meijer3, Mathias Müller14 and Birgit Strobl1*

Author Affiliations

1 Institute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria

2 Center for Medical Research, Medical University of Graz, Graz, Austria

3 Department of Cell Biology and Genetics, ErasmusMC, Rotterdam, Netherlands

4 Biomodels Austria, University of Veterinary Medicine Vienna, Vienna, Austria

5 Department of Forensic Medicine, Medical University of Vienna, Austria

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BMC Cell Biology 2010, 11:61  doi:10.1186/1471-2121-11-61

Published: 5 August 2010

Additional files

Additional file 1:

Oct-6 protein is expressed in pMEFs in response to IFNβ treatment. Bandshift assays including supershifts with α-Oct-1, α-Oct-2 and α-Oct-6 antibodies and Oct-6-/- MEFs as controls.

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Additional file 2:

Expression of Oct-6 during MCMV infection is largely dependent on type I IFN and Jak/Stat signalling. Bandshift assays of whole cell extracts from WT, IFNβ-/-, Ifnar1-/-, Tyk2-/-, and Stat1-/- macrophages infected with MCMV.

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Additional file 3:

Oct-6 DNA-binding activity in response to IFNβ or poly(I:C) treatment in foetal liver- and bone marrow-derived macrophages.

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Additional file 4:

Overexpression of Oct-6 in pMEFs enhances the expression of IFNβ and IFNα mRNAs, but does not influence the expression of Egr2 and Pmp22 mRNAs.

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Additional file 5:

Absence of Oct-6 does not influence the expression patterns of panIFNα, IFNβ, Egr2 and Pmp22 mRNAs upon DNA transfection in MEFs. Comparison of transfected WT and Oct6-deficient foetal liver-derived macrophages.

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Additional file 6:

Absence of Oct-6 in macrophages has no impact on MCMV replication at a MOI of 0.1. Comparison of MCMV replication in WT and Oct-6-deficient foetal liver-derived macrophages at a lower MOI.

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Additional file 7:

Differentially expressed genes as determined by microarray analysis that could be functionally annotated (96 out of 200; WT vs. Oct-6-/-, at least 2-fold difference, p < 0.05).

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Additional file 8:

Complete list of differentially expressed genes (WT vs. Oct-6-/-, at least 2-fold difference, p < 0.05).

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Additional file 9:

3A (sheet 1): Gene Ontology categories up-regulated by poly(I:C) treatment in WT cells (p < 0.05); 3B (sheet 2): Gene Ontology categories down-regulated by poly(I:C) treatment in WT cells (p < 0.05).

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