Cell viability after freezing and thawing within the i3C and in control standard cryo-vials. U937 cell populations underwent slow or fast freezing followed by thawing in either the i3C or cryo-vials, after which they were exposed, while residing in the i3C or the vial, to staining mixture of FDA and PI. The percentages of dead U937 cells (PI positive) are shown. Bars represent mean and SD values for at least 6 experiments. p < 0.05.
Deutsch et al. BMC Cell Biology 2010 11:54 doi:10.1186/1471-2121-11-54