Monitoring cell viability after the freezing (slow cooling rate, 1°C per minute) and thawing cycle. Representative transmitted light (A) and corresponding fluorescent images of FDA (B) and PI (C) staining of U937 cells within the i3C after a freezing and thawing cycle. The red line connects images of the only cell in the field found to be PI-positive. The blue arrow indicates a reference region in the picowell field at the bottom of the i3C chamber. Bars: 25 μm.
Deutsch et al. BMC Cell Biology 2010 11:54 doi:10.1186/1471-2121-11-54