Additional file 2.

Isolation of MLMVECs from mouse lungs by FACS sorting. Low passage (passage 3) MLMVECs were sorted by FACS into CD31+/Lyve1+ (gate R1) and CD31+/Lyve1- (gate R2) cells for further subculturing and immunophenotyping. The percentage of cells in the different regions is indicated (%).

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Schniedermann et al. BMC Cell Biology 2010 11:50   doi:10.1186/1471-2121-11-50