In vivo studies of mouse lung EPCs after lentiviral transduction with GFP marker gene. A) Cells as a monolayer (left) or as spheroids (right) used for Matrigel implantation into mice. B) De novo formation of lymphatic vessels as revealed by double staining with anti-GFP and anti-podoplanin (which is LEC-specific) in combination with nuclear Dapi staining. Note that the diagonal vessel in the picture expresses both markers. C) De novo formation of blood vessels as revealed by double staining with anti-GFP and anti-CD31 (strong CD31 staining is characteristic for BECs) in combination with nuclear Dapi staining. Erythrocytes in the vessels display green/yellow background fluorescence.
Schniedermann et al. BMC Cell Biology 2010 11:50 doi:10.1186/1471-2121-11-50