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Open Access Research article

Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters

Dan Bi*, Fu Guo Chen*, Wen Jie Zhang, Guang Dong Zhou, Lei Cui, Wei Liu and Yilin Cao

Author Affiliations

Department of Plastic and Reconstructive Surgery, Shanghai 9thPeople's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China

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BMC Cell Biology 2010, 11:46  doi:10.1186/1471-2121-11-46

Published: 25 June 2010

Abstract

Background

We have previously obtained a clonal population of cells from human foreskin that is able to differentiate into mesodermal, ectodermal and endodermal progenies. It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells.

Results

Sixty-one single-cell-derived dermal fibroblast clones were established from human foreskin by limiting dilution culture. Of these, two clones could be differentiated into neuron-, adipocyte- or hepatocyte-like cells under certain culture conditions. In addition, those two clones were able to differentiate into islet-like clusters under pancreatic induction. Insulin, glucagon and somatostatin were detectable at the mRNA and protein levels after induction. Moreover, the islet-like clusters could release insulin in response to glucose in vitro.

Conclusions

This is the first study to demonstrate that dermal fibroblasts can differentiate into insulin-producing cells without genetic manipulation. This may offer a safer cell source for future stem cell-based therapies.