Figure 4.

α-N-terminal methylation of RCC1α is not affected by the stable association of Ran. U2OS cells were co-transfected with GFP, RCC1α-GFP or RCC1αK4Q-GFP and mCherry, mCherry-RanWT or mCherry-RanT24N. Cell lysates were analysed by Western blotting for α-dimethylated RCC1, RCC1, Ran and actin as a loading control. Methylated RCC1α-GFP was revealed on a light exposure of the α-dimethylated RCC1 blot and endogenous RCC1 isoforms on a dark exposure. A non-specific reactive band noted by [18] is indicated by *. The migration position of molecular mass markers (kDa) are shown left.

Hitakomate et al. BMC Cell Biology 2010 11:43   doi:10.1186/1471-2121-11-43
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