Subcellular localization of RNF122. (A) HeLa or HEK293T cells were grown on coverslips and transfected with RNF122-N1 and CAML-FLAG. To inhibit the degradation of RNF122-N1, the cells were treated with 2.5 μM MG132 for 6 h. The cells were then treated with anti-FLAG antibody and FITC-conjugated goat anti-mouse antibody, and observed under a confocal microscopy. The results showed that the cytoplasmic distribution of CAML entirely overlapped that of RNF122. (B) HEK293T cells were transfected with RNF122-N1 and RNF122ΔTM-N1 and observed under a fluorescence microscope.
Peng et al. BMC Cell Biology 2010 11:41 doi:10.1186/1471-2121-11-41