Differentiation decreases p42/44 and PKB signaling whereas S6K activity increases. A) p44/42 MAPK Thr202/Tyr204 phosphorylation relative to total p44/42 (p-p44/42), B) PKB Thr308 phosphorylation relative to total PKB (p-PKB) and C) S6K1 phosphorylation and activity determined relative to total S6K1 3 hrs post plating (subconfluent; SC), 24 h post plating (Day 0), 24 h after achieving confluence (Day 1), or 72 hrs following differentiation (Day 3). * indicates significantly different from SC, † indicates significantly different from Day 1. Values were obtained by normalising specific phosphorylation to total protein content as described in methods.
Hamilton et al. BMC Cell Biology 2010 11:37 doi:10.1186/1471-2121-11-37