Molecular mechanisms involved in Angiotensin II-induced PlGF upregulation in EA.Hy 926 cells and HUASMCs. (A) Effects of different inhibitors on the PlGF mRNA expression induced by Ang II by using Quantitative RT-PCR. Cells were pretreated with different inhibitors for 30 mins and stimulated with Ang II (10-6 mol/L) for 24 hours, and PlGF mRNA expression was assayed by Q-PCR (n = 3 in each group). * P < 0.05 vs control cells. # P < 0.05 vs cells treated with Ang II alone. (B) Effects of different inhibitors on the PlGF protein expression induced by Ang II detected by using ELISA. PlGF protein contents in the cell culture media were assayed by ELISA (n = 3 in each group). * P < 0.05 vs control cells. # P < 0.05 vs cells treated with Ang II alone.
Pan et al. BMC Cell Biology 2010 11:36 doi:10.1186/1471-2121-11-36