Effects of Sal B on cell viability and toxicology in HK-2 cells. (A) Cell viability was determined by the alamarBlue assay. The results were expressed as a percentage in the reduction of alamarBlue. 1 μM and 10 μM of Sal B had no significant effect (P > 0.05) on cell viability during each incubation time period, whereas 100 μM of Sal B decreased the viability of HK-2 cells by 11% (P < 0.05) and 20% (P < 0.05), when compared with control cells after 12 h and 24 h of incubation, respectively. (B) Cell toxicology was determined by high content screening (HCS) assay. HK-2 cells were plated in a 96-well plate at a density of 4,000 cells/well. Cells were incubated with 1-100 μM of Sal B for 24 h and then stained with 1 × MPCT1 Fluor Dye. Cell images from the various fluorescent dye stains were taken by HCS (×200). There were no obvious changes in nuclear morphology, membrane permeability, or lysosomal mass/pH after incubation with 1 μM and 10 μM of Sal B when compared with control cells. However, 100 μM of Sal B presented as an obvious cytotoxicity to cells. As shown by the indicator dye, there are fewer nuclei and an increase in membrane permeability.
Wang et al. BMC Cell Biology 2010 11:31 doi:10.1186/1471-2121-11-31