Sal B down-regulated MMP-2 and MMP-9 activation induced by TGF-β1 in HK-2 cells. HK-2 cells were incubated with serum-free medium or treated with (1) 2.5 ng/ml TGF-β1, (2) 2.5 ng/ml TGF-β1 plus 1 μM Sal B, (3) 2.5 ng/ml TGF-β1 plus 10 μM Sal B, (4) 2.5 ng/ml TGF-β1 plus 10 μM SB-431542 for 24 h. The activation of MMP-2 and MMP-9 derived from cells in the conditioned media were analyzed by gelatin zymographic analysis. The results show that TGF-β1 induced pro-MMP-2, the activation of pro-MMP-2, and MMP-9 secretion. 1 μM Sal B, 10 μM Sal B, and SB-431542 significantly decreased the secretion of these MMPs after being induced by TGF-β1. The values are as shown as folds vs. Control. **P < 0.01 vs. control; # #P < 0.01 vs. TGF-β1.
Wang et al. BMC Cell Biology 2010 11:31 doi:10.1186/1471-2121-11-31