Additional file 4.
Mimosine treatment reduces the level of bound PCNA from S phase cells. BJ1 cells were grown in 10% or 0.03% serum in the presence and absence of 120 μg/ml mimosine. After 8 h, treated cells were extracted with Triton X-100 and fixed in methanol (see Methods) before staining for cytometry. Control cells were harvested at time zero. Red dots = mitotic cells. SS = serum starvation.
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Frisa and Jacobberger BMC Cell Biology 2010 11:26 doi:10.1186/1471-2121-11-26