Additional file 1.
Western blot detection of tightly bound proteins. BJ1 cells were extracted with 0.5% Triton X-100 before solubilization for electrophoresis. 105 cells were loaded in each lane. Western blots were probed with antibodies to the indicated proteins as described in Methods. (A) the two antibodies used in the study. (B) antibodies to two other replication complex proteins and cyclin B1, which does not appear to be tightly bound at a detectable level in asynchronously growing cells. In addition to detection of tightly bound antigen, these blots also demonstrate the high specificity of the Mcm6 and PCNA antibodies, since cross reacting bands are not evident.
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Frisa and Jacobberger BMC Cell Biology 2010 11:26 doi:10.1186/1471-2121-11-26