Cells in the low Mcm6 cluster express loaded Mcm6. BJ1 cells were harvested at 0 and 24 hours after serum starvation and prepared for electrophoresis after extracting the cells with Triton X-100 and washing. Equal cell numbers were loaded. The immunoblots were developed on X-Ray film that were then imaged and quantified. At 24 h, Mcm6 intensity was reduced by 25% and PCNA intensity by 72%. Under the conditions for serum starving BJ1 cells, there was an average of 5% of cells in S, G2 and M.
Frisa and Jacobberger BMC Cell Biology 2010 11:26 doi:10.1186/1471-2121-11-26