Table 1

Cell speed variability caused by imprecise centroid selection

Cell type

σ in μm

original MD

AMD in μm/min

%

AMD (smoothed)

%


sc

3

0.181

0.423

234

0.125

69

sc

4.5

0.578

320

0.151

84

sc

6

0.747

414

0.181

100

sc

7.5

0.921

510

0.218

121


mc

3

0.622

0.723

116

0.465

75

mc

4.5

0.829

133

0.473

76

mc

6

0.956

154

0.484

78

mc

7.5

1.099

177

0.499

80


fc

3

1.787

1.821

102

1.589

89

fc

4.5

1.860

104

1.591

89

fc

6

1.923

108

1.597

89

fc

7.5

1.985

111

1.598

89


Three cell types where tested (slow cell (sc), medium fast cell (mc), fast cell (fc)). The centroid positions where artificially varied within a standard deviation (σ, both axes) of 3 to 7.5 μm around the real centroid and the average mean displacement (AMD) computed for each set of varied tracks (200 tracks per setting). Variation of centroid positions resulted in overestimation of cell speeds, which was most pronounced for the slowest cell. Smoothing of "noisy" cell tracks by a centered moving average filter (window size 5) tended to underestimate MD values to varying degrees.

Huth et al. BMC Cell Biology 2010 11:24   doi:10.1186/1471-2121-11-24

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