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Significantly improved precision of cell migration analysis in time-lapse video microscopy through use of a fully automated tracking system

Johannes Huth12, Malte Buchholz2, Johann M Kraus1, Martin Schmucker1, Götz von Wichert3, Denis Krndija3, Thomas Seufferlein34, Thomas M Gress2 and Hans A Kestler13*

Author Affiliations

1 Research group of Bioinformatics and Systems Biology, Institute of Neural Information Processing, Ulm University, Albert-Einstein-Allee 11, D-89081 Ulm, Germany

2 Department of Gastroenterology and Endocrinology, University Hospital of Marburg, Germany

3 Clinic of Internal Medicine I, Medical Centre Ulm University, Albert-Einstein-Allee 23, D-89081 Ulm, Germany

4 Department of Internal Medicine I, Martin-Luther-University, Halle-Wittenberg, Germany

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BMC Cell Biology 2010, 11:24  doi:10.1186/1471-2121-11-24

Published: 8 April 2010

Additional files

Additional file 1:

Supplementary information: Core elements of the tracking system.

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Additional file 2:

Supplementary information: Manual of the TimeLapseAnalyzer.

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Additional file 3:

Supplementary Video File A. Example video file of automatically tracked untreated Panc1 cancer cells, recorded with the Differential Interference Contrast (DIC) imaging technique. Cell tracks (cell paths) are marked with colored spots. Green flashing spots indicate a cell division; red flashing spots indicate either a track loss or the leaving of a cell out of the field of view (event near the border). In addition, each track is also plotted into the last video frame for a final overview.

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Additional file 4:

Supplementary Video File B. A second example video file of automatically tracked untreated Panc1 cancer cells, recorded with the DIC imaging technique. Cell tracks (cell paths) are marked with colored spots. Green flashing spots indicate a cell division; red flashing spots indicate either a track loss or the leaving of a cell out of the field of view (event near the border). In addition, each track is also plotted into the last video frame for a final overview.

Format: AVI Size: 2.4MB Download file

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Open Data

Additional file 5:

Supplementary Video File C. Example video file of automatically tracked untreated Hela cancer cells, recorded with the Phase Contrast (PC) imaging technique. Cell tracks (cell paths) are marked with colored spots. Green flashing spots indicate a cell division; red flashing spots indicate either a track loss or the leaving of a cell out of the field of view (event near the border). In addition, each track is also plotted into the last video frame for a final overview.

Format: AVI Size: 3.3MB Download file

Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more

Open Data