Mild impairment of the repair of UV induced DNA lesions by abrogation of expression of endogenous CC3. (A). Expression of CC3 in MCF10A cells infected with a control lentivirus and a CC3 siRNA expressing virus. (B). MCF10A cells were subjected to UVC irradiation at 10 J/m2, and harvested at different times after exposure. Relative repair of CPD lesions was quantified using ELISA with an anti-CPD antibody. Data represent average of three independent experiments. (C). A representative Southwestern blot analysis of DNA from UV-exposed MCF7 cells with anti-CPD antibody. (D) CPD repair in MCF7 cells, control and with silenced CC3. All charts show the average and standard deviation of three individual experiments.
Fong et al. BMC Cell Biology 2010 11:23 doi:10.1186/1471-2121-11-23