Host cell reactivation of the UV-damaged plasmid in presence of exogenously expressed CC3. (A). HeLa cells were transfected as described in Methods with reporter plasmids and either 1× or 2× amount of effector plasmids indicated. Fold HCR in presence of control, wild type CC3 and mutant CC3 plasmids is shown. P values were determined by t-test, n = 3. Shown are the statistical significances of differences seen between 1× amount of CC3 versus 1× amount vector (0.064), 1× mutant CC3 versus vector (0.264) and for 2× amounts of CC3 versus vector (0.0065) and mutant/vector (0.211). (B). Expression of CC3 protein in U373neo and stably transfected U373cc3 cells determined by Western blot analysis of cell extracts using antibody to CC3 . Antibody to hnRNPI was used for loading control. (C). U373 cell clones, control (U373neo) and CC3 expressing (U373cc3) were transfected with luciferase reporter plasmids and analyzed for HCR. All results are average of three transfections performed in duplicates.
Fong et al. BMC Cell Biology 2010 11:23 doi:10.1186/1471-2121-11-23