Additional file 4.

Comparison of the threshold-based translocation assay and the distribution of translocation in a cell population. Reporter cell lines were activated with TNF╬▒ (10 ng/ml) for the indicated time, fixed, stained with Hoechst and analyzed with automated microscopy. Top four rows show indicated parameters for all cells in one well per time point (approximately 300-1000 cells). Bottom row shows the results of the threshold-based translocation assay shown in Figure 2 and Additional file 3. Error bars = SD of duplicates.

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Bartfeld et al. BMC Cell Biology 2010 11:21   doi:10.1186/1471-2121-11-21