H. pylori-induced oscillations of p65 nuclear translocation at the population level are dependent on MOI in simulations and experiments. a) Mathematical model for simulating p65 oscillations: Stimulus induces degradation of IκBα (1), IκBα competitively inhibits binding of p65 to DNA (2) and transcription and translation is simplified by an activation delay (3). b) Mathematical model predicts oscillations of nuclear p65 (green) and protein levels of IκBα (red) upon stimulation (grey) at the single-cell level. c) Simulation of population-level p65 oscillations after H. pylori infections at an MOI 100 and 1. Oscillations of p65 nuclear translocation within 500 individual cells were simulated (grey lines) and percentages of cells with nuclear p65 (blue line) were determined by a threshold set for nuclear p65. d) Experimental confirmation of predicted population-level oscillations of p65 (at MOI 100 and 1). AGS SIB02 cells were infected with H. pylori at the indicated MOI, fixed, analyzed by automated microscopy and then the percentage of cells with nuclear p65-GFP was determined, as described in Figure 2. Error bars = SD of experiment performed in triplicates. Results are representative of three independent experiments.
Bartfeld et al. BMC Cell Biology 2010 11:21 doi:10.1186/1471-2121-11-21