Endogenous Bmp2 is detectable in the nuclei of three cultured cell lines. (a) Non-transfected 10T1/2 mesenchymal cells, BALB/3T3 fibroblasts, and RCS cells were cultured on slides and immunostained using an anti-Bmp2 antibody (green). Nuclei were stained with TO-PRO-3 (red), and cells were examined by laser confocal microscopy. (b) Antibody specificity was verified by preabsorbing anti-Bmp2 antibody with recombinant human BMP-2 before immunostaining.
Felin et al. BMC Cell Biology 2010 11:20 doi:10.1186/1471-2121-11-20