Strain-does dependent effect on adipogenesis in ADSCs. A. Oil-Red O staining of adult ADSCs that were subjected to AS of various strain magnitudes for the first 48 h and then adipogenic medium alone for the next 8 days. The non-strain cells (0%) were adipogenically induced for 10 days. Scale bars = 500 μm. B. AP2 and PPARγ expression in ADSCs subjected to various AS as in A. The result shown was the expression levels normalized to β-actin and to the non-strain samples as in Figure 8. **: P < 0.01, *: P < 0.05, N = 3, compared ΔΔCT to non-strain and 0.5% strain groups.
Huang et al. BMC Cell Biology 2010 11:18 doi:10.1186/1471-2121-11-18