Direct interaction of Yck1/2 with the glucose sensors is not required for degradation of Mth1. (A) Subcellular localization of GFP-Yck1 and GFP-Yck2 expressed in wild type (BY4742) and akr1Δ mutant (JKY56) strains was examined by fluorescence microscopy. (B) Western blot analysis of GFP-Mth1 in wild-type and akr1Δ mutant strains. Yeast cells were grown as described in Figure 2.
Pasula et al. BMC Cell Biology 2010 11:17 doi:10.1186/1471-2121-11-17