Mth1 does not shuttle between the nucleus and cytoplasm. (A) Images of GFP-Mth1 expressing yeast cells grown in galactose (2%) before and after photobleaching in the indicated region. One of two foci (arrow) was bleached and then recovery of the fluorescence in the bleached zone was monitored. Time is in seconds after photobleaching. (B) Time-lapse observation of Mth1 degradation in high-glucose medium (4%). Numbers indicate time in minutes. Yeast cells, wild-type (WT; BY4742) and grr1 mutant (grr1Δ; YM4783), expressing GFP-Mth1 were observed under the Zeiss LSM 510 META Confocal Laser Scanning Microscope (CLSM).
Pasula et al. BMC Cell Biology 2010 11:17 doi:10.1186/1471-2121-11-17