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Open Access Highly Accessed Research article

Integrin-linked kinase functions as a downstream signal of platelet-derived growth factor to regulate actin polymerization and vascular smooth muscle cell migration

Mitra Esfandiarei1*, Sahar Abdoli Yazdi1, Virginia Gray2, Shoukat Dedhar2 and Cornelis van Breemen1

Author Affiliations

1 Child & Family Research Institute, Department of Anesthesiology, Pharmacology, and Therapeutics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4, Canada

2 Department of Cancer Genetics, British Columbia Cancer Research Centre, University of British Columbia, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada

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BMC Cell Biology 2010, 11:16  doi:10.1186/1471-2121-11-16

Published: 23 February 2010

Additional files

Additional file 1:

Figure 1S. Effect of PDGF treatment on mouse aortic SMCs migration and proliferation. Mouse SMCs were treated with increasing doses of PDGF-BB for 24 hours and cell migration and proliferation were measured. Cells treated with 10% serum were used as the positive control. As shown, 25 ng/ml of PDGF-BB significantly increased SMCs migration (3 fold increase) with no effect on cell proliferation indicating that the observed increase in cell migration is not due to the proliferatory effect of PDGF-BB in SMC culture. Data is representative of three independent experiments (n = 3 of each culture condition).

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Additional file 2:

Figure 2S. Kinetics of MAPKs activation in mouse aortic SMC culture. PDGF-BB treatment resulted in phosphorylation and activation of all three members of MAPKs family in mouse aortic SMCs. Data represents three independent experiments.

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Additional file 3:

Figure 3S. PDGF induces LIMK and cofilin phosphorylation in mouse aortic SMCs. Aortic SMCs were serum starved overnight and then treated with 25 ng/ml of PDGF-BB. Cell lysates were collected in various timepoints and phosphorylation of LIMK and cofilin was measured. Data represents three independent experiments.

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