Translocation of S100A11 into the nucleus of human U-2 OS cells after stress stimulation. (A) Fixed human HaCaT keratinocytes treated with bleomycin (BLM) for 30 min were immunostained with anti-S100A11 antibody. In BLM treated cells decreased staining in the cytoplasm as well as increased staining in the nucelus can be observed. (B) U-2 OS cells transfected with GFP-S100A11 were treated with CaCl2 (10 mM) or BLM (12.5 IU/ml), and analyzed by immunostaining followed by laser scanning microscopy for GFP-S100A11 and for γH2AX at different time points at indicated.
Gorsler et al. BMC Cell Biology 2010 11:100 doi:10.1186/1471-2121-11-100