Additional file 4.
Recombinant expression of Ha-Ntf2 and Ha-Ran in E. coli and specificity of antibodies. Recombinant expression of Ha-Ntf2 and Ha-Ran in E. coli and specificity of antibodies. A and C, analysis of recombinant expression of Ha-Ntf2 and Ha-Ran by 12.5% SDS-PAGE; B and D, examining the specificity of the antibody against Ha-Ntf2 and Ha-Ran by immunoblotting, arrow indicate the target protein from 5-36 h fat bodies. Lane 1, E. coli proteins with pET30a-Ha-Ntf2 or pET30a-Ha-Ran without induction; lane 2, E. coli proteins with pET30a-Ha-Ntf2 or pET30a-Ha-Ran induced by IPTG; lane 3, lysate supernate; lane 4, lysate precipitate; lane 5, purified recombinant protein by nickel affinity chromatography; lane 6, standard protein marker.
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He et al. BMC Cell Biology 2010 11:1 doi:10.1186/1471-2121-11-1