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Open Access Research article

Function of nuclear transport factor 2 and Ran in the 20E signal transduction pathway in the cotton bollworm, Helicoverpa armigera

Hong-Juan He1, Qian Wang12, Wei-Wei Zheng1, Jin-Xing Wang1, Qi-Sheng Song2 and Xiao-Fan Zhao1*

Author Affiliations

1 School of Life Sciences, the Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, Shandong University, Jinan 250100, Shandong, PR China

2 Division of Plant Sciences, University of Missouri, Columbia, Missouri 65211, USA

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BMC Cell Biology 2010, 11:1  doi:10.1186/1471-2121-11-1

Published: 2 January 2010

Additional files

Additional file 1:

Full-length cDNA sequences and predicted amino acid sequences of Ha-Ntf2. Full-length cDNA sequences and predicted amino acid sequences of Ha-Ntf2. Amino acid residues in real line are casein kinase II phosphorylation sites.

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Open Data

Additional file 2:

Full-length cDNA sequences and predicted amino acid sequences of Ha-Ran. Full-length cDNA sequences and predicted amino acid sequences of Ha-Ran. Overstriking amino acid residues are GTP-binding nuclear protein Ran signatures; Boxed amino acid residues are protein kinase C phosphorylation sites. The shadowed sequence is an ATP/GTP-binding site motif A. Poly A tail adding signal is in broad brush.

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Open Data

Additional file 3:

Multiple alignments of Ha-Ntf2 and Ha-Ran with other insects. Multiple alignments of Ha-Ntf2 and Ha-Ran with other insects. D. melanogaster [Ntf2, AAS98195.1; Ran: NP_651969.1]; A. aegypti [Ntf2, AAS79346.1; Ran, EAT38849.1]; A. mellifera [Ntf2, XP_392921.1; Ran, XP_393761.1]; M. hirsutus [ABM55654.1]; B. mori [NP_001040274.1]; H. armigera [Ntf2, DQ875254; Ran, EU860296]. The shadow regions in black are conserved sequences.

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Open Data

Additional file 4:

Recombinant expression of Ha-Ntf2 and Ha-Ran in E. coli and specificity of antibodies. Recombinant expression of Ha-Ntf2 and Ha-Ran in E. coli and specificity of antibodies. A and C, analysis of recombinant expression of Ha-Ntf2 and Ha-Ran by 12.5% SDS-PAGE; B and D, examining the specificity of the antibody against Ha-Ntf2 and Ha-Ran by immunoblotting, arrow indicate the target protein from 5-36 h fat bodies. Lane 1, E. coli proteins with pET30a-Ha-Ntf2 or pET30a-Ha-Ran without induction; lane 2, E. coli proteins with pET30a-Ha-Ntf2 or pET30a-Ha-Ran induced by IPTG; lane 3, lysate supernate; lane 4, lysate precipitate; lane 5, purified recombinant protein by nickel affinity chromatography; lane 6, standard protein marker.

Format: TIFF Size: 423KB Download file

Open Data