K18 interacts with LRP16 in yeast cells. Yeast AH109 cells were transformed with the indicated GAL4-DBD (DNA Binding Domain) and GAL4-AD (Activation Domain) chimeric constructs and β-galactosidase activity was measured by a liquid o-nitrophenyl-β-D-galactoside (ONPG) assay. The experiment was repeated 3 times, and 2 different yeast transformants were used for each measurement. The interaction of p53 with SV40 large T-antigen protein provided a positive control.
Meng et al. BMC Cell Biology 2009 10:96 doi:10.1186/1471-2121-10-96