Table 1

Effect of various substances on the cell surface expression of wild type (HA-WT) and mutant (HA-MM) HA-ABCA1 variants

Drug

Known effect

ABCA1 cell surface expression

HA-WT

HA-MM


ALLN

Calpain inhibitor

⇧ (+ 60%)

⇧ (+ 30%)


ApoA-I

cholesterol acceptor

⇧ (+ 30%)

no effect


Atorvastatin

cholesterol level lowering

no effect

no effect


Brefeldin A

trans-Golgi transport inhibitor

⇩ (- 60%)

⇩ (- 40%)


Cycloheximide

protein synthesis inhibitor

⇩ (- 30%) (viability ⇩)

⇩(- 30%) (viability ⇩)


Cyclosporin A

immunosuppressor

⇧ (+ 60%)

⇧ (+ 60%)


Ezetimibe

inhibitor of intestinal cholesterol absorption

⇩ (- 20%)

no effect


Glyburide

ABCA1 inhibitor

no effect

no effect


Niacin

cholesterol level lowering

no effect

no effect


Nifedipine

Ca2+-channel blocker

no effect

no effect


Methyl-β-cyclodextrin

cholesterol depletion

no effect (viability ⇩)

no effect (viability ⇩)


Cholesterol-methyl-β-cyclodextrin

cholesterol overloading

no effect (viability ⇧)

no effect (viability ⇧)


Verapamil

Ca2+-channel blocker

no effect

no effect


Kasza et al. BMC Cell Biology 2009 10:93   doi:10.1186/1471-2121-10-93

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