Open Access Research article

Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction

Florian Böhrnsen13, Ulrich Lindner3, Markus Meier3, Abdelalim Gadallah1, Peter Schlenke2, Hendrik Lehnert3, Jürgen Rohwedel1 and Jan Kramer13*

Author Affiliations

1 Institute of Virology and Cell Biology, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

2 Institute for Transfusion Medicine and Transplantation Immunology, University of Münster, Domagkstraße 11, 48149 Münster, Germany

3 Medical Department I, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

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BMC Cell Biology 2009, 10:92  doi:10.1186/1471-2121-10-92

Published: 19 December 2009

Additional files

Additional file 1:

The karyotype of mesenchymal progenitor cells from bone marrow up to passage 15 (A) as well as from perirenal adipose tissue (B) and mediastinal tissue (C) up to passage 13 is demonstrated by G-banding.

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Additional file 2:

Monolayer and MMS cultivation result in comparable concentrations of RNA during adipogenic (A) and osteogenic (B) differentiation. In contrast to the MMB cultivation technique the chondrogenic differentiation of murine mesenchymal progenitors via the MMS system results in a continuous increase of the content of RNA (C). Mean values ± SED derived from at least three independent experiments (n = 3) are shown. Significant differences are indicated: * = p ≤ 0,05; *** = p ≤ 0,001.

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Additional file 3:

Description: Mesenchymal progenitor cells derived from murine bone marrow (BM), perirenal adipose tissue (PAT), and mediastinal stromal tissue (MST) do not show differentiation via MMB (A) and MMS (B) without application of specific induction media. Expression of collagen type II and X as well as of bone sialoprotein (BSP) and osteopontin (OP) were analyzed by immunostaining, and these results serve as additional negative controls. Nuclei are stained with DAPI (blue). DIC = differential interference contrast.

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