Autophagosome formation expressing GFP-LC3 puncta in Bβ2 and Bβ1 clones. The parental SK-N-SH cells and Bβ1 and Bβ2 clones were transfected with GFP-LC3 plasmid for 48 h and observed under the fluorescence microscope for GFP (green) followed by counter-staining with DAPI(blue). Puncta fluorescence in GFP-LC3 transfected cells can be detected in cells of both Bβ1 and Bβ2 clones. Bars, 20 μm.
Cheng et al. BMC Cell Biology 2009 10:91 doi:10.1186/1471-2121-10-91