Table 3

ADP-kinetics of respiration in permeabilized fibres in fish R-solution

Temp

Creatine

n

V0

Vmax

ACR

KM ADP


10°C

-

6

2.34 ± 0.21

24.85 ± 3.44

10.68 ± 1.22

783 ± 160

+

6

2.01 ± 0.19

22.6 ± 3.83

11.03 ± 1.62

376 ± 55 *

15°C

-

5

2.78 ± 0.32

29.13 ± 3.16

10.66 ± 1.01

579 ± 64

+

5

2.51 ± 0.08

27.25 ± 2.22

10.96 ± 1.11

301 ± 42 *

20°C

-

5

3.35 ± 0.07

28.06 ± 3.37

8.40 ± 1.05

293 ± 115

+

5

3.37 ± 0.15

30.59 ± 1.83

9.10 ± 0.52

224 ± 42


Notation and statistical tests are as in Table 1. * denotes P < 0.05 significantly different in the presence of creatine. Temperature had a significant effect on V0 (P < 0.05 and P < 0.001 in the absence and presence of creatine, respectively) and KM ADP in the absence of creatine (P < 0.05).

Sokolova et al. BMC Cell Biology 2009 10:90   doi:10.1186/1471-2121-10-90

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